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Detection of boldenone, methandriol and testosterone in equine urine by GC-MS

Diğer Başlık: Detection of boldenone, methandriol and testosterone in equine urine by GC-MS

Oluşturulma Tarihi: 1999

Niteleme Bilgileri

Tür: Makale

Yayınlanma Durumu: Yayınlanmış

Dosya Biçimi: Word

Dil: İngilizce

Konu(lar): Tarım (Genel),

Revizyon: 1

Yazar(lar):

Emeği Geçen(ler): Keskin, Fevziye İpek (Editör),


Yayınlayan: Etlik Merkez Veteriner Kontrol ve Araştırma Enstitüsü Yayın Adı: Bulletin 559 62 nd Meeting of the Association of Official Chemists Yayın Tarihi: 1999 Sayı: 1 Cilt: 559 Yayınlandığı Sayfalar: 17


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Anahtar Kelimeler Boldenone, methandriol, testosterone, equine urine
Özet Anabolic androgenic steroids are synthetic derivatives of testosterone, which is the primary male sex hormone, or a related compound that is derived from (or similar in structure and action to) this hormone. They are an important class of performance enhancing drug in horse racing and other sports. That’s why using of them in racehorses is prohibited in many racing jurisdictions around the world. This paper describes an analytical method developed for the detection of anabolic androgenic steroids boldenone, methandriol and testosterone in equine urine. The equine urine fortified with the steroid standards. The steroids were extracted from equine urine with dichloromethane at pH 6.8. The extracts were analysed by Agilent 6890N-5973 Gas Chromatography coupled to Mass Spectrometry (GC-MS) using 12 m L x 0.20 mm ID, 0.33 µm particle size HP 1 column. The method was validated for methandriol. Extraction recovery, limit of detection and limit of quantification were 90%, 3.54 ng/ml and 11.8 ng/ml, respectively.This results point out that the method’s recovery is extremely high, reproducible and sensitive.
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Notlar Bahrke, M. S., Yesalis, C. E. (2004) Abuse of anabolic androgenic steroids and related substances in sport and exercise. Curr. Opin. Pharmacol. 4, 614-620.Dehennin, L., Petit, E., Bonnaire, Y., Bruyas, J.-F., Bizec, B. L., Plou, P. (2007) Urinary excretion of 5(10)-estrene-3?,17?-diol and estrone by the female horse: Complementary indicators of early pregnancy screened with regard to a putative anabolic doping practice. J. Steroid Biochem. Mol. Biol. 104, 85-91.Dumasia, M. C., Houghton, E., Sinkins, S. (1986) Development of a gas chromatographic-mass spectrometric method using multiple analytes for the confirmatory analysis of anabolic steroids in horse urine : I. Detection of testosterone phenylpropionate administrations to equine male castrates. J. Chromatogr. B Biomed Sci Appl. 377, 23-33. Guan, F., Uboh, C. E., Soma, L. R., Luo, Y., Rudy, J., Tobin, T. (2005) Detection, quantification and confirmation of anabolic steroids in equine plasma by liquid chromatography and tandem mass spectrometry. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 829, 56-68.Ho, E. N. M., Yiu, K. C. H., Tang, F. P. W., Dehennin, L., Plou, P., Bonnaire, Y., Wan, T. S. M. (2004) Detection of endogenous boldenone in the entire male horses. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 808, 287-294.Ho, E. N. M., Leung, D. K. K., Wan, T. S. M., Yu, N. H. (2005) Metabolic studies of methenolone acetate in horses. Analytica Chimica Acta. 540, 111-119.Ho, E. N. M., Kwok, W. H., Leung, D. K. K., Wan, T. S. M., Wong, A. S. Y. (2007a) Metabolic studies of turinabol in horses. Analytica Chimica Acta. 586, 208-216.Ho, E. N. M., Leung, D. K. K., Leung, G. N. W., Wan, T. S. M., Wong, H. N. C., Xu, X., Yeung, J. H. K. (2007b) Metabolic studies of mesterolone in horses. Analytica Chimica Acta. 596, 149-155.Holt, R. I. G., Mulligan, I. E., Sönksen, P. H. (2009) The history of doping and growth hormone abuse in sport. Growth Horm. IGF Res. 19, 320-326.Houghton, E., Dumasia, M. C., Teale, P., Smith, S. J., Cox, J., MarshaII, D., Gower, D. B. (1990) The use of stable isotopes and gas chromatography/mass spectrometry in the identification of steroid metabolites in the equine. Steroids. 55, 433-439.Hungerford, N. L., Sortais, B., Smart, C. G., McKinney, A. R., Ridley, D. D., Stenhouse, A. M., Suann, C. J., Munn, K. J., Sillence, M. N., McLeod, M. D. (2005) Analysis of anabolic steroids in the horse: development of a generic ELISA for the screening of 17?-alkyl anabolic steroid metabolites. J. Steroid Biochem. Mol. Biol. 96, 317-334. Kaya, S., Bilgili, A. (2002) Doping için kullanılan maddeler, Veteriner Hekimliğinde Toksikoloji, Eds: S. Kaya, İ. Pirinçci, A. Bilgili, Baskı 2, Medisan Yayınevi, Ankara. pp 603-616.Kaya, S. (2007) Üremeyi etkilyen hormonlar, Veteriner Farmakoloji, Ed: S. Kaya, Cilt 2, Baskı 4, Medisan Yayınevi, Ankara. pp 63-83.Kintz, P., Cirimele, V., Toulet, V. D., Villain, M., Ludes, B. (2001) Doping control for methenolone using hair analysis by gas chromatography-tandem mass spectrometry. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 766, 161-167.Lenehan, P. (2004) What are anabolic steroids?, Social histroy of anabolic steroids, Anabolic agents, Boldenone undeconoate (injetable), Anabolic Steroids and Other Performance Enhancing Drugs. Taylor & Francis, e-Library. pp 2-107. MacAuley, D. (1996) Drugs in sport. BMJ. 313, 211-215.Parker, J. N., Parker, P. M. (2002) What is anabolic steroid abuse?, The Official Patient’s Sourcebook on Anabolic Steroid Dependence, Eds: J. N. Parker, P. M. Parker, Icon Health Publications, USA. pp 12.Roig, M., Segura, J., Ventura, R. (2007) Quantitation of 17?-nandrolone metabolites in boar and horse urine by gas chromatography-mass spectrometry. Analytica Chimica Acta. 586, 184-195.Singh, A. K., Gordon, B., Hewetson, D., Granley, K., Ashraf, M., Mishra, U., Dombrovskis, D. (1989) Screening of steroids in horse urine and plasma by using electron impact and chemical ionization gas chromatography-mass spectrometry. J. Chromatogr. A. 479, 233-242.Steroid (2009) Anabolic Steroids Profile Methandriol. [Online]. Available: http://www.steroid.com/methandriol.php [08.10.2009].Yu, N. H., Ho, E. N. M., Leung, D. K. K., Wan, T. S. M. (2005) Screening of anabolic steroids in horse urine by liquid chromatography-tandem mass spectrometry. J. Pharm. Biomed. Anal. 37, 1031-1038.

KaynakçaBahrke, M. S., Yesalis, C. E. (2004) Abuse of anabolic androgenic steroids and related substances in sport and exercise. Curr. Opin. Pharmacol. 4, 614-620.Dehennin, L., Petit, E., Bonnaire, Y., Bruyas, J.-F., Bizec, B. L., Plou, P. (2007) Urinary excretion of 5(10)-estrene-3?,17?-diol and estrone by the female horse: Complementary indicators of early pregnancy screened with regard to a putative anabolic doping practice. J. Steroid Biochem. Mol. Biol. 104, 85-91.Dumasia, M. C., Houghton, E., Sinkins, S. (1986) Development of a gas chromatographic-mass spectrometric method using multiple analytes for the confirmatory analysis of anabolic steroids in horse urine : I. Detection of testosterone phenylpropionate administrations to equine male castrates. J. Chromatogr. B Biomed Sci Appl. 377, 23-33. Guan, F., Uboh, C. E., Soma, L. R., Luo, Y., Rudy, J., Tobin, T. (2005) Detection, quantification and confirmation of anabolic steroids in equine plasma by liquid chromatography and tandem mass spectrometry. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 829, 56-68.Ho, E. N. M., Yiu, K. C. H., Tang, F. P. W., Dehennin, L., Plou, P., Bonnaire, Y., Wan, T. S. M. (2004) Detection of endogenous boldenone in the entire male horses. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 808, 287-294.Ho, E. N. M., Leung, D. K. K., Wan, T. S. M., Yu, N. H. (2005) Metabolic studies of methenolone acetate in horses. Analytica Chimica Acta. 540, 111-119.Ho, E. N. M., Kwok, W. H., Leung, D. K. K., Wan, T. S. M., Wong, A. S. Y. (2007a) Metabolic studies of turinabol in horses. Analytica Chimica Acta. 586, 208-216.Ho, E. N. M., Leung, D. K. K., Leung, G. N. W., Wan, T. S. M., Wong, H. N. C., Xu, X., Yeung, J. H. K. (2007b) Metabolic studies of mesterolone in horses. Analytica Chimica Acta. 596, 149-155.Holt, R. I. G., Mulligan, I. E., Sönksen, P. H. (2009) The history of doping and growth hormone abuse in sport. Growth Horm. IGF Res. 19, 320-326.Houghton, E., Dumasia, M. C., Teale, P., Smith, S. J., Cox, J., MarshaII, D., Gower, D. B. (1990) The use of stable isotopes and gas chromatography/mass spectrometry in the identification of steroid metabolites in the equine. Steroids. 55, 433-439.Hungerford, N. L., Sortais, B., Smart, C. G., McKinney, A. R., Ridley, D. D., Stenhouse, A. M., Suann, C. J., Munn, K. J., Sillence, M. N., McLeod, M. D. (2005) Analysis of anabolic steroids in the horse: development of a generic ELISA for the screening of 17?-alkyl anabolic steroid metabolites. J. Steroid Biochem. Mol. Biol. 96, 317-334. Kaya, S., Bilgili, A. (2002) Doping için kullanılan maddeler, Veteriner Hekimliğinde Toksikoloji, Eds: S. Kaya, İ. Pirinçci, A. Bilgili, Baskı 2, Medisan Yayınevi, Ankara. pp 603-616.Kaya, S. (2007) Üremeyi etkilyen hormonlar, Veteriner Farmakoloji, Ed: S. Kaya, Cilt 2, Baskı 4, Medisan Yayınevi, Ankara. pp 63-83.Kintz, P., Cirimele, V., Toulet, V. D., Villain, M., Ludes, B. (2001) Doping control for methenolone using hair analysis by gas chromatography-tandem mass spectrometry. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 766, 161-167.Lenehan, P. (2004) What are anabolic steroids?, Social histroy of anabolic steroids, Anabolic agents, Boldenone undeconoate (injetable), Anabolic Steroids and Other Performance Enhancing Drugs. Taylor & Francis, e-Library. pp 2-107. MacAuley, D. (1996) Drugs in sport. BMJ. 313, 211-215.Parker, J. N., Parker, P. M. (2002) What is anabolic steroid abuse?, The Official Patient’s Sourcebook on Anabolic Steroid Dependence, Eds: J. N. Parker, P. M. Parker, Icon Health Publications, USA. pp 12.Roig, M., Segura, J., Ventura, R. (2007) Quantitation of 17?-nandrolone metabolites in boar and horse urine by gas chromatography-mass spectrometry. Analytica Chimica Acta. 586, 184-195.Singh, A. K., Gordon, B., Hewetson, D., Granley, K., Ashraf, M., Mishra, U., Dombrovskis, D. (1989) Screening of steroids in horse urine and plasma by using electron impact and chemical ionization gas chromatography-mass spectrometry. J. Chromatogr. A. 479, 233-242.Steroid (2009) Anabolic Steroids Profile Methandriol. [Online]. Available: http://www.steroid.com/methandriol.php [08.10.2009].Yu, N. H., Ho, E. N. M., Leung, D. K. K., Wan, T. S. M. (2005) Screening of anabolic steroids in horse urine by liquid chromatography-tandem mass spectrometry. J. Pharm. Biomed. Anal. 37, 1031-1038.


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